Electrocardiographic parameters were measured in 28 free-ranging roe deer (Capreolus capreolus). The electrocardiograms (ECGs) were recorded in base-apex lead, standard bipolar limb leads (I, II, III), and augmented unipolar limb leads (aVR, aVL, aVF). Morphology and amplitude of P waves, QRS complexes, and T waves were analyzed in all seven leads. Cardiac rhythm, heart rate, and durations of P, QRS, and T waves, PR interval, QTc interval, and ST segment were calculated in the base-apex lead. The mean electrical axis for each individual was determined from the net amplitude of the QRS complex in leads I and III. All of the animals had a sinus rhythm. Heart rates ranged from 60–180 beats per minute, with a mean ± SD of 104.8 ± 44.1. The electrical axis was 100°–220°, with a mean ± SD of 169.5° ± 40.8°. The base-apex lead provided the most uniform ECG patterns and higher mean amplitudes than did standard leads.